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Journal of the Korean Society of Traumatology 2008;21(2):120-127.
Time Course of Inducible NOS Expression of Lung Tissue during Sepsis in a Rat Model
Joong Hee Kim, Seong Chun Kim, Woon Yong Kwon, Gil Joon Suh, Yeo Kyu Youn
1Department of Emergency Medicine, Seoul National University College of Medicine, Korea.
2Department of Emergency Medicine, Gyeongsang National University Hospital, Korea. gsimem@medimail.co.kr
3Department of Surgery, Seoul National University College of Medicine, Korea.
백서의 패혈증 모델에서 시간에 따른
폐조직에서의 Inducible Nitric Oxide Synthase 발현
김중희·김성춘1·권운용·서길준·윤여규2
서울대학교 의과대학 응급의학교실, 경상대학교병원 응급의학과1
서울대학교 의과대학 외과학교실2
Abstract
PURPOSE
Many studies on the time course of inducible nitric oxide synthase (iNOS) gene expression have been performed in the LPS (Lipopolysaccharide)-induced endotoxemic model, but there have been few experimental approaches to continuous peritonitis-induced sepsis model. We conducted this study to establish basic data for future sepsis-related research by investigating the time course of iNOS gene expression and the relationship with the production of inflammatory mediators in the early sepsis model induced by cecal ligation and puncture (CLP).
METHODS
Male Sprague-Dawley rats were operated on by sing the CLP method to induce of peritonitis; and then, they were sacrificed and samples of blood and lung tissues were obtained at various times (1,2,3,6,9 and 12 h after CLP). We observed the expression of iNOS mRNA from lung tissues and measured the synthesis of nitric oxide, IL-1beta , and TNF-alpha from the blood.
RESULTS
iNOS mRNA began to be expressed at 3 h and was maintained untill 12 h after CLP. The nitric oxide concentration was increased significantly at 6 h, reached its peak level at 9 h, and maintained a plateau untill 12 h after CLP. TNF-alpha began to be detected at 3 h, increased gradually, and decreased steeply from 9 h after CLP. IL-1beta showed its peak level at 6 h after CLP, and tended to decrease without significance.
CONCLUSION
We observed that the iNOS gene was expressed later in peritonitis-induced sepsis than in LPSinduced sepsis. Nitric oxide and key inflammatory mediators were also expressed later in peritonitis-induced sepsis than in LPS-induced sepsis.
Key Words: Nitric oxide synthase; Inflammation mediators; Sepsis; Peritonitis
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